宫颈癌中端粒酶的表达和高危型人乳头瘤病毒感染

目的:研究不同程度子宫颈病变中高危型人乳头瘤病毒HR-HPV感染和端粒酶活性的表达,以探讨两者在宫颈癌及宫颈上皮内瘤变中的作用及相关性。方法:采用第二代杂交捕获技术检测宫颈脱落细胞HPV-DNA含量,并用免疫组织化学EnVision二步法检测宫颈组织标本中端粒酶的表达。结果:(1)端粒酶阳性表达率在对照组、CINⅠ、CINⅡ、CINⅢ和宫颈癌组分别为10.00%、16.67%、40.00%、70.00%、95.00%,宫颈癌组高于CINⅢ,CINⅢ高于CINⅡ,CINⅡ高于CINⅠ,差异均有统计学意义(x2=4.329,P=0.037;x2=4.327,P=0.038;x2=4.022,P=0.045)。(2)随着宫颈病变级别的增加,高危型HPV的阳性率和病毒负荷量均增高。高危型HPV的阳性率在宫颈癌和CINⅢ组明显高于对照组、CINⅠ及CINⅡ(x2=29.501~7.414,P<0.01)。高危型HPV的病毒负荷量在对照组与其他4组比较,差异均有统计学意义(P<0.05);CINⅠ组分别与CINⅡ、CINⅢ及宫颈癌组比较差异均有统计学意义(P<0.05)。(3)随着宫颈病变级别的增加,高危型HPV的阳性率和端粒酶阳性表达率依次递增,两者有明显的相关性(r=0.943,P<0.01)。结论:高危型HPV感染和端粒酶活性均与宫颈癌前病变及宫颈癌的发生发展密切相关,有望作为子宫颈癌前病变和宫颈癌筛查的监测指标。

Expression of Telomerase and Infection of High Risk Human Papillpmavirus in Cervical Cancer

Objective: To investigate the effect and relationship between high risk human papillomavirus(HR-HPV) infection and telomerase expression in the development of cervical carcinoma.Methods: HPV DNA in cervical exfoliated cells was detected by sec-ond-generation hybird capture technology,then the expression of telomerase was evaluated by immunohistochemistry.Results:(1) The positive rate of telomerase in control group,cervical intraepithelial neoplasia(CIN)Ⅰ group,CINⅡ group,CINⅢ group and cervical car-cinoma group was 10.00 %,16.67 %,40.00 %,70.00 % and 95.00 %,respectively.The positive rate of telomerase in cervical cancer was significantly higher than that in CINⅢ(x2=4.329,P=0.037).And the positive rate in CINⅢ was significantly higher than that in CINⅡ(x2=4.327,P=0.038).And the positive rate in CINⅡ was significantly higher than that in CINⅠ(x2=4.022,P=0.045).(2) With the de-terioration of carcinoma lesions,HR-HPV load and the positive rate of HPV infection increased gradually.The positive rate of HPV in-fection was significantly different between the cervical cancer group,CINⅢ group and CINⅡgroup,CINⅠgroup and control group(x2=29.501~7.414,P<0.01).The high risk HPV load was significantly different between control group and the other groups(P<0.05).(3) With the increase of the cervical lesion level,both the high-risk HPV detection rate and the telomerase positive expression rate in-creased,and there was an obvious correlation between the HPV infection and the telomerase expression(r=0.943,P<0.01).Conclusion: High-risk type HPV infection and the expression of telomerase had an obvious correlation with the development of cervical intraepithelial neoplasia and cervical cancer,and might be a monitoring index to detect and predict cervical cancer.