| 小鼠巨噬细胞RAW264.7 的培养技巧及经验总结 |
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| 引用本文： | 方瑶毛旭虎. 小鼠巨噬细胞RAW264.7 的培养技巧及经验总结[J]. 现代生物医学进展, 2012, 12(22): 4358-4359 |
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| 作者姓名： | 方瑶毛旭虎 |
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| 作者单位： | 第三军医大学医学检验系临床微生物教研室 重庆400038 |
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| 基金项目： | 国家重大科技专项(2008ZXJ09014-007) |
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| 摘 要： | RAW264.7细胞具有很强的黏附和吞噬抗原的能力,是研究微生物学、免疫学的常用细胞株.很多研究者发现这种细胞形态极不稳定,细胞状态的评价也很困难.本文作者结合RAW264.7培养经历及文献资料探讨RAW264.7细胞培养的经验教训和评价细胞状态的方法,旨在为培养该细胞的科研工作者提供一定的借鉴.
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| 关 键 词： | 小鼠巨噬细胞RAW264.7(TIB-71) 细胞培养 细胞形态 |
Culture Skill and Experience of RAW264.7 |
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| FANG Yao,MAO Xu-hu. Culture Skill and Experience of RAW264.7[J]. Progress in Modern Biomedicine, 2012, 12(22): 4358-4359 |
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| Authors: | FANG Yao MAO Xu-hu |
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| Affiliation: | (Department of Clinical Microbiology and Immunity,the Third Military Medical University,Chongqing,400038,China) |
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| Abstract: | RAW264.7 has doughty capability of adhesion and antigen phagocytosis,so it is commonly used in microbiology and immunology.However,many researchers find that the cell is very unstable because of its morphous variation,and it is difficult to value its state.In this paper we mainly discussed the skills and experience in culturing RAW264.7 and method of evaluating cell state.We hope to provide some references to researchers who would culture such cell line. |
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| Keywords: | Mice macrophages RAW264.7(TIB-71) Cell culture Cell morpha |
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