海洋放线菌Streptomyces variabilis strain 6-1次级代谢产物的研究

目的:对来自海洋软珊瑚的链霉菌6-1(Streptomyces variabilis strain 6-1)进行次级代谢产物的分离和鉴定,寻找具有生物活性的化合物,为人类健康服务。方法:采用液体培养基对分自海洋软珊瑚Scleronephthya sp中的链霉菌6-1(Streptomyces variabi-lis strain 6-1)进行发酵培养,用乙酸乙酯对发酵液进行萃取;采用半制备高效液相色谱(semi-preparative HPLC)分离方法对乙酸乙酯萃取物进行分离纯化,得到单体化合物;运用电喷雾质谱(ESI-MS)、核磁共氢振(1H NMR)、核磁共振碳谱(13C NMR)和物理性质对所得单体化合物进行结构鉴定。结果:从海洋链霉菌6-1(strain 6-1)发酵液的乙酸乙酯萃取物中分离得到3个单体化合物,分别鉴定为:7,4'-二羟基异黄酮(1)、5,7,4'-三羟基异黄酮(2)和丁烯酸内酯-Ⅰ(3)。结论:丁烯酸内酯-Ⅰ是从链霉菌属首次分离得到,化合物1和2均是从Streptomyces variabilis中首次分离得到;变异链霉菌6-1(Streptomyces variabilis strain 6-1)可以作为活性化合物3(丁烯酸内酯-Ⅰ)的重要来源。

Study on the SecondaryMetabolites from a Marine-derived Actinomycete Streptomyces Variabilis Strain 6-1*

Objective: To isolate and identify the secondary metabolites produced by Streptomyces variabilis strain 6-1 associated with marine soft coral Scleronephthya sp.and to search bioactive compounds,serving for people’s health.Methods: The actinomycete Streptomyces variabilis strain 6-1 isolated from the soft coral Scleronephthya sp.was fermented in the liquid medium,the fermentation broth was extracted by acetic ether.The ethyl acetate extract of the fermentation broth was separated on semi-preparative HPLC to afford pure compounds.Structure of these compounds were identified by ESI-MS,1H NMR and 13C NMR spectroscopy methods and physical data.Results: The three compounds isolated from fermentation broth of the microorganism were elucidated as 7,4’-dihydroxy isoflavone(1),5,7,4’-trihydroxy isoflavone(2),butyrolactone-I(3).Conclusions: Compound 3 was first isolated from fermentation broth of Streptomyces,compound 1 and 2 were first isolated from fermentation broth of Streptomyces variabilis.;marine actinobacterium Streptomyces variabilis strain 6-1 is a good source of active compound 3(butyrolactone-I).