不同剂量rhBMP-2/CPC 体内骨诱导活性的比较研究*

目的:对比不同剂量rhBMP-2与多孔CPC复合后的诱导成骨效应,探讨与多孔CPC复合后的rhBMP-2的量效关系.方法:将0.5 mg/ml、1 mg/ml、2 mg/ml、3 mg/ml 4种不同剂量的rhBMP-2与多孔CPC材料复合后,植入36只小鼠双侧股部肌肉内,分别于术后1周、2周及4周取材,通过大体观察、组织学分析、形态计量学分析、荧光双标测定,观察4组诱导成骨情况.结果:植入1周,rhBMP-2与多孔CPC材料复合表现出了较明显的剂量依赖性,含有较多rhBMP-2的材料内诱导形成的骨组织也较多,但骨组织的增加并未随着rhBMP-2剂量的增加而连续递增,2 mg组和3 mg组新生骨组织含量无明显差异(P＞0.05).植入4周,新生骨组织向材料内部生长,但此时的新生骨组织面积较2周增加不显著(P＞0.05).0.5 mg组新生骨组织含量仍处于最低水平,而其它三组之间却无明显差异(P＞0.05).结论:在0.5 mg/ml-2.0 mg/ml剂量范围,与多孔CPC复合的rhBMP-2诱导成骨量与其剂量成正比,最佳剂量为2 mg/ml.

Effects of Porpous CPC with Different Concentrations of rhBMP -2 on Inductive Osteogenesis in Vivo*

Objective: To investigate the biological effect of inductive osteogenesis of porous CPC with different dose of rhBMP-2 and the interrelation to the dose of rhBMP-2.Methods: The porous CPC with 0.5 mg/ml,1 mg/ml,2 mg/ml,3 mg/ml rhBMP-2 were respectively implanted into the muscle of 36 mice.The mice were killed at 1,2 and 4 weeks postoperation.The biological effect of inductive osteogenesis was observed by histology,morphometry and fluorescence.Results: At 1 week,the dose dependent of rhBMP-2 could be observed obviously.The better ossification was observed in the porous CPC with more rhBMP-2.But the newly formed bone did not increase continously with the increase of rhBMP-2.There were no significant difference in newly formed bone between 2 mg/ml group and 3mg/ml group(P>0.05).At 4 weeks,newly formed bone had grown into the porous CPC.But there were no significant difference in the area of newly formed bone at 4 weeks compared with that at 2 weeks(P>0.05).The content of new bone in 0.5 mg/ml group was significantly lower than that in other groups.There were no obvious differences in content of new bone among 1 mg/l,2 mg/l and 3mg/ml groups(P>0.05).Conclusions: The content of new bone increases with the increasing in the dose of rhBMP-2 in the dose cope of 0.5mg/ml-2.0mg/ml,and the optimal dose of rhBMP-2 is 2.0mg/ml.