HPLC 法测定不同来源槐角中槐角苷含量

目的:建立HPLC法测定槐角中槐角苷含量测定方法并检测不同来源槐角中槐角苷的含量。方法:Luna 5u C18(2)100A柱(250 mm×4.60 mm,5μm),柱温为35℃,流动相为甲醇-乙腈-1%冰醋酸(40:5:55),流速为1.0 ml/min,检测波长为260 nm。结果:槐角苷质量浓度在14.58 mg/L～53.46 mg/L范围内呈良好的线性关系,回归方程为y=110353x-274717(r=0.9999),平均回收率为99.5%,RSD为1.27%;不同来源槐角中槐角苷含量范围4.27%～8.78%。结论:该方法简便、快速、灵敏、可靠;不同来源的槐角中槐角苷含量有较大差异,其中以陕北黄土高原品种含量最高,可为槐角的质量保证提供依据。

Determination of Sophoricoside Content in Fructus Sophorae from DifferentSources by HPLC

Objective: To develop a HPLC method for the determination of sophoricoside content in Fructus Sophorae and to de-termine the content of sophoricoside in Fructus Sophorae from different sources.Methods: The extracts of Fructus sophorae were separat-ed in a Luna 5u C18(2)100A column(250 mm x 4.60 mm,5 μm);Methanol-acetonitrile-1 % glacial acetic acid(40:5:55) was used as mobile phase;The flow rate was 1.0 ml/min;The efluents were monitored at 260 mm.Results: The linear ranges of sophoricoside were 14.58 mg/L～53.46 mg/L.The average recovery rate for sophoricoside was 99.5%,while the RSD was 1.27%.The content of sophoricoside ranges from 4.27 % to 8.78 %.Conclusions: The method is simple,rapid,sensitive and reliable;The content of sophoricoside in Fructus Sophorae from different sources was different and the content of sophoricoside was higher in the Fructus Sophorae of Shanbei Loess Plateau than other places.