A genome-wide analysis of wide compatibility in rice and the precise location of the S5 locus in the molecular map |
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Authors: | K D Liu J Wang H B Li C G Xu A M Liu X H Li Q Zhang |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China, CN;(2) Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China, CN |
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Abstract: | The discovery of wide-compatibility varieties (WCVs) that are able to produce normal fertility hybrids when crossed both
to indica and japonica rice has enabled the fertility barrier between indica and japonica subspecies to be broken and provided
the possibility of developing inter-subspecific hybrids in rice breeding programs. However, a considerable variation in the
fertility level of hybrids from the same WCV crossed to different varieties has often been observed. One hypothesis for this
variable fertility is that additional genes are involved in hybrid fertility besides the wide-compatibility gene (WCG). To
assess such a possibility, we performed a genome-wide analysis by assaying a large population from a three-way cross ‘02428’/‘Nanjing
11’//‘Balilla’ using a total of 171 RFLP probes detecting 191 polymorphic loci distributed throughout the entire rice linkage
map. Our analysis recovered 3 loci conferring significant effects on hybrid fertility. The major locus on chromosome 6 coincided
in chromosomal location with the previously identified S
5
locus, and the 2 minor loci that mapped to chromosomes 2 and 12, respectively, were apparently distinct from all previously
reported hybrid sterility genes. Interaction between the indica and japonica alleles at each of the loci caused a reduction
in hybrid fertility. The joint effect of the 2 minor loci could lead to partial sterility even in the presence of the WCG.
The location of the S
5
locus on the molecular marker linkage map was determined to be approximately 1.0 cM from the RFLP locus R2349. This tight
linkage will be useful for marker-aided transfer of the WCG in hybrid rice breeding and for map-based cloning.
Received: 5 February 1997 / Accepted: 4 April 1997 |
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Keywords: | Oryza sativa L Indica and japonica Hybrid sterility Mapping Rice breeding |
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