Effect of differentiation on the regulation of indole alkaloid production in Catharanthus roseus hairy roots |
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Authors: | O A Moreno-Valenzuela R M Galaz-Avalos Y Minero-García V M Loyola-Vargas |
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Institution: | (1) Unidad de Biología Experimental, Centro de Investigación Científica de Yucatán, Apdo. Postal 87, Cordemex 97310, Mérida, Yucatán, México Fax: (99)-813900 e-mail: vmloyola@cicy.cicy.mx, XX |
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Abstract: | In vitro cultures of hairy root derived from Catharanthus roseus accumulate higher levels of indole alkaloids than cell suspension cultures. Hairy roots were interconverted to undifferentiated
cells by manipulation of the culture medium. When the concentration of micronutrients in the culture medium was five times
that of Phillips and Collins (1979) medium, cell suspensions formed from the hairy roots. The alkaloid content was five times
lower in the cell suspensions than in the control, but upon regeneration of the roots the alkaloid content regained its original
level. The formation of cell suspensions from hairy roots was also accompanied by a reduction in tryptophan decarboxylase
and the strictosidine synthase activity to less than 5% and 30%, respectively. 3-Hydroxymethylglutaryl coenzyme A reductase
activity was the same in the cell suspension and in the regenerated line.
Received: 12 February 1998 / Revision received: 21 May 1998 / Accepted: 5 June 1998 |
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Keywords: | Catharanthus roseus Differentiation Hairy roots Tryptophan decarboxylase Strictosidine synthase 3-hydroxymethylglutaryl coenzyme A reductase |
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