Salicylic acid improves podophyllotoxin production in cell cultures of Linum album by increasing the expression of genes related with its biosynthesis |
| |
| Authors: | Morteza Yousefzadi Mozafar Sharifi Mehrdad Behmanesh Alireza Ghasempour Elisabeth Moyano and Javier Palazon |
| |
| Institution: | (1) Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-154, Tehran, Iran;(2) Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran;(3) Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran;(4) Departament de Ciencies de la Salut i la Vida, Universitat Pompeu Fabra, Av. Dr. Aiguader 80, 08003 Barcelona, Spain;(5) Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, Av. Joan XXII, sn. 08028 Barcelona, Spain; |
| |
| Abstract: | Treatment of Linum album cell cultures with 10 μM salicylic acid (SA) for 3 days improved podophyllotoxin (PTOX) production up to 333 μg/g dry weight
(DW): over three times that of the control cultures. qPCR analyses showed that in SA-treated cells, the expression of the
genes coding for phenylalanine ammonia-lyase (PAL), cinnamoyl-CoA reductase (CCR) and cinnamyl-alcohol dehydrogenase (CAD), all involved in the first steps of PTOX biosynthesis, also increased reaching a peak 8–12 h after the treatment. Expression
of the pinoresinol-lariciresinol reductase gene (PLR), which is involved in one of the last biosynthetic steps, was not affected by SA. The selective action of SA on these genes
can be applied to control the biotechnological production of this anticancer agent. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
