Protein synthesis during oxygen conformance and severe hypoxia in the mouse muscle cell line C2C12 |
| |
Authors: | Arthur P G Giles J J Wakeford C M |
| |
Affiliation: | Department of Biochemistry, The University of Western Australia, Nedlands, W.A., Australia. parthur@cyllene.uwa.edu.au |
| |
Abstract: | Oxygen conformance can be described as the ability to reduce energy demand, and hence oxygen consumption, in response to a decline in oxygen availability without a decrease in the concentration of ATP. It has been proposed that oxygen conformance may enhance cellular survival at low oxygen concentrations. We demonstrate that non-contracting C2C12 cells, a mouse skeletal muscle cell line, are capable of oxygen conformance. Typically, we found oxygen consumption to decline by 30-40% as the concentration of oxygen was reduced from 100 microM to 10 microM. Unexpectedly, the rate of protein synthesis, a major energy consumer in the cell, did not decrease significantly during oxygen conformance. Unlike oxygen conformance, severe hypoxia (<0.5 microM) caused a 36% decline in the concentration of PCr, and under these conditions of energy stress, the rate of protein synthesis declined by 43%. We conclude that there are two distinct metabolic responses to declines in oxygen concentration in non-contracting C2C12 cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|