Sex-specific binding and inactivation of agglutination factor in Chlamydomonas eugametos |
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Authors: | H. L. A. Fijst F. A. Ossendorp P. van Egmond A. M. I. E. Kamps A. Musgrave H. van den Ende |
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Affiliation: | (1) Plantenfysiologisch Laboratorium, Universiteit van Amsterdam, Kruislaan 318, NL-1098 SM Amsterdam, The Netherlands |
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Abstract: | Gametes of opposite mating type (mt+ and mt-) of the green alga Chlamydomonas eugametos agglutinate via their flagella as a prelude to sexual fusion. To quantitate sexual agglutination, an in vitro assay has been developed using 35S-labeled flagella and the isolated mt-agglutination factor. It is shown that not only isolated flagella, but also the mt-agglutination factor rapidly bind to the flagella of intact gametes of the opposite mating type. This confirms the role of the mt-agglutination factor in determining the sexual agglutinability of mt-gametes. As a function of binding, the agglutinative power of the flagella of both mating types is destroyed by a temperature-sensitive process. Likewise, the mt-agglutination factor can be completely inactivated.Abbreviations Mt+/- mating type plus or minus - PAS periodic-acid Schiff-reagent - Hepes 4-(2-hydroxyethyl)-1-piperazineethansulfonic acid - HMC buffer Hepes buffer (10 mM. pH 7.2, containing 1 mM MgCl2 and 1 mM CaCl2) |
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Keywords: | Chlamydomonas (gametes) Flagellum (mating type) Glycoprotein Sexual agglutination (gametes) |
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