Cytogenetic localization of 44 new coding sequences in the horse |
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Authors: | Sophie Godard Anne Vaiman Laurent Schibler Denis Mariat Daniel Vaiman Edmond-Paul Cribiu Gérard Guérin |
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Institution: | (1) INRA, Centre de Recherches de Jouy, Laboratoire de Génétique biochimique et de Cytogénétique, Département de Génétique Animale, INRA, 78352 Jouy-en-Josas cedex, France, FR |
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Abstract: | The purpose of this study was to increase the number of genes assigned by in situ hybridization to equine chromosomes and
thus the number of links for comparative mapping with other species. Forty-four new sequences were added to the horse cytogenetic
map by FISH mapping of BAC clones containing genes (35) or ESTs (9). Three approaches were developed: use of horse BAC clones
screened with (i) horse EST primers, (ii) interspecific consensus intraexonic primers, and (iii) use of goat BAC containing
genes previously localized on goat chromosomes. Present data suggest that the second approach is the most promising. A total
of 46 segments containing one or several genes could be compared, among which 40 loci could be included in 16 synteny groups
between human and horse, displaying one ordered segment and several breaking points along chromosomes. All single BAC localizations
confirm the most recent mapping data. Twenty-six out of 31 chromosomes now contain a gene mapped by in situ hybridization,
and 14 new arm-to-arm segment homologies were revealed.
Received: 2 May 2000 / Accepted: 27 July 2000 |
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