The EnvZ protein of Salmonella typhimurium LT-2 and Escherichia coli K-12 is located in the cytoplasmic membrane |
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| Authors: | Peter Liljeströ m |
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| Affiliation: | Molecular Genetics Laboratory, Department of Genetics, University of Helsinki, Arkadiankatu 7, SF-00100 Helsinki, Finland |
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| Abstract: | Abstract The osmoregulated expression of the porin proteins OmpC and OmpF in S. typhimurium and E. coli is dependent on the regulatory proteins OmpR and EnvZ. The function of the EnvZ protein is not clear. In order to establish the cellular location of EnvZ two different methods of buoyant sucrose density centrifugation was employed. The presence of EnvZ in the different fractions was visualised by immunoblotting. It was conclusively shown that the EnvZ protein is located in the cytoplasmic membrane fraction. The result is in agreement with the available sequence data which shows that the EnvZ polypeptide contains two long hydrophobic stretches. |
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| Keywords: | Salmonella typhimurium Escherichia coli EnvZ protein cytoplasmic membrane |
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