Quantitative analysis of biofilm EPS uronic acid content |
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Authors: | Mojica Kristina Elsey Danielle Cooney Michael J |
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Institution: | Hawaii Natural Energy Institute, School of Ocean and Earth Sciences and Technology, University of Hawaii, Honolulu HI 96822, United States. |
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Abstract: | The uronic acids assay was evaluated for its ability to measure the amount of uronic acids contained within a biofilm exopolysaccharide matrix. Cytophaga lytica, a marine bacterium isolated from a naturally occurring biofilm, was used to form single-species biofilms for the method assessment. The assay was found to be simple, reproducible, and sensitive to 1 microg levels, suggesting its potential for application as a screening technique for compounds that inhibit the production of microbial biofilm exopolysaccharide containing uronic acids. |
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