Expression of recombinant DNA functional products in Escherichia coli anucleate minicells |
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| Authors: | Khachatourians G G Berezowsky C M |
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| Affiliation: | Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, Canada. |
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| Abstract: | This review covers the use of anucleate minicells of Escherichia coli for expressing the recombinant DNA encoded proteins. We briefly discuss the methods being used for preparation of anucleate minicells, incorporation of cloned DNA and assessment of gene expression. While the largest use has been that of microbially derived cloned functional DNA, examples of eukaryotic gene product synthesis have also been reviewed. This technology may represent some interesting commercial opportunities. |
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