Constant fed-batch culture of methanol-utilizing corynebacterium producing vitamin B 12

Summary A fed-batch culture of methanol-utilizing microorganism (Corynebacterium sp. XG), a vitamin B 12 producer, was carried out with constant feed of substrate. Experimental results agreed with the theorical model proposed in the literature. Using this feeding system, the final biomass and vitamin B 12 concentration reached 16.3 g/l and 880 g/l respectively after a 53 h incubation period at 30 °C.