| 固定化蛋白质A在流动注射荧光免疫分析中的应用 |
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| 引用本文： | 任学贞,J.N.MILLER.固定化蛋白质A在流动注射荧光免疫分析中的应用[J].生物化学与生物物理进展,1995,22(1):80-84. |
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| 作者姓名： | 任学贞 J.N.MILLER |
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| 作者单位： | 山东大学化学系;Department of Chemistry, Loughborough University of Technology, Loughborough, U.K |
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| 摘 要： | 利用蛋白质A在近中性pH条件下，能够与大多数哺乳动物抗体的Fc部分结合；在低pH条件下，又能将抗体洗脱下来的特性，以一蛋白质A固相免疫反应器，分离与抗体结合及未结合的抗原.并结合流动注射进样方便的特点建立了一个快速、简便并易于自动化的流动注射荧光免疫分析方法.研究了各种实验变量对测定的影响，并用于人体转铁蛋白的测定．
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| 关 键 词： | 蛋白质A 荧光免疫分析 流动注射 转铁蛋白 |
| 收稿时间： | 1994/1/18 0:00:00 |
| 修稿时间： | 1994/3/21 0:00:00 |
Application of Immobilized Protein A in Flow Injection Fluoroimmunoassay |
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| Ren Xuezhen and J.N.MILLER.Application of Immobilized Protein A in Flow Injection Fluoroimmunoassay[J].Progress In Biochemistry and Biophysics,1995,22(1):80-84. |
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| Authors: | Ren Xuezhen and JNMILLER |
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| Institution: | Department of Chemistry, Shandong University;Department of Chemistry, Loughborough University of Technology, Loughborough, U.K |
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| Abstract: | A convenient,semi-automated flow injection fluoroimmunoassay method has been developed incorporating the flexibility of sample handling of flow injection system containing a immobilized protein A immunoreactor. Protein A can binds the Fc region of most mammlian antibodies at near neutral pH,and the bound antibodies can be eluted from protein A at acid pH.This peculiar property of protein was exploided to separate the antigen bound to antibody and the unbound ones.Experimental variables have been studied and the method has been used to determine the transferrin contents in human serum. |
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| Keywords: | protein A fluoroimmunassay flow injection transferrin |
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