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Heavy-ion beam irradiation is an effective technique for reducing major allergens in peanut seeds |
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| Authors: | Cerrone S Cabanos Hiroki Katayama Hiroyuki Urabe Chikara Kuwata Yuri Murota Tomoko Abe Yutaka Okumoto Nobuyuki Maruyama |
| Institution: | 1. Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611-0011, Japan 2. Chiba Prefectural Agriculture and Forestry Research Center, Upload Crops Breeding Laboratory, Chiba, Japan 3. Chiba Prefectural Agriculture and Forestry Research Center, Biotechnology Laboratory, Chiba, Japan 4. Radiation Biology Team, RIKEN Nishina Center, RIKEN, Hirosawa, Wako, 351-0198, Japan 5. Laboratory of Plant Breeding, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan |
| Abstract: | Heavy-ion beam irradiation is an effective technique for mutation breeding to produce new cultivars. Heavy-ion beams have high linear energy transfer capable of breaking the double-stranded DNA molecules, thus inducing stable knockout mutants. Here, we report the first application of this technology to produce hypoallergenic peanut lacking major allergens, Ara h 2 and 3, from the Japanese Nakateyutaka variety. After irradiation with either N or C heavy-ion beams at a dose of 100?Gy, seventeen knockout mutants from 11,335 screened M2 seeds were obtained, eight of which lacked either one of the two isoforms of Ara h 2, and the other nine lacked one of the isoforms of Ara h 3. This result indicates that heavy-ion beam irradiation is a powerful means of producing knockout hypoallergenic peanuts. |
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