DNA strand scission by enzymically generated oxygen radicals |
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| Authors: | K Brawn I Fridovich |
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| Institution: | Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710 U.S.A. |
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| Abstract: | Col E1 DNA suffers strand scission when exposed to xanthine oxidase acting aerobically on xanthine. Strand scission was prevented by low levels of superoxide dismutase or of catalase. Mannitol, benzoate, or histidine, which scavenge OH · but which react with neither O2? nor H2O2, also prevented strand scission. Replacement of 0.1 mm ethylenediaminetetraacetate by 0.1 mm diethylenetriaminepentaacetate prevented strand scission. Three mechanisms for the production of OH ·, or of a comparably powerful oxidant, by metal-catalyzed interaction of O2? with H2O2, are proposed. |
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| Keywords: | To whom reprint requests and correspondence should be addressed |
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