Comparison of bone inductive proteins of rat and porcine bone matrix |
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| Authors: | N Muthukumaran T K Sampath A H Reddi |
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| Affiliation: | 1. National Key Laboratory of Chinese Medicine Modernization, State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China;2. Haihe Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China;3. Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China;1. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China;2. State Key Laboratory of Bioreactor Engineering, East China University of Scienceand Technology, Shanghai 200237, PR China;1. Sbarro Health Research Organization, Temple University, Philadelphia, PA, USA;2. Resis Srl, 10010 Samone, Italy;3. Northwestern University, Evanston, IL, USA;4. Howard Hughes Medical Institute, Chevy Chase, MD, USA;5. Karolinska Institutet, Stockholm, Sweden;6. University of Turin, Tornio, Italy;7. Accademia Nazionale dei Lincei, Rome, Italy;8. RIKEN Center for Integrative Medical Sciences, Yokohama, Japan;9. Toscana Life Sciences Foundation, Siena, Italy;10. University of Modena and Reggio Emilia, Modena, Italy;11. University of Oxford, Oxford, UK;12. University of Dundee, Dundee, UK;13. University of Salerno, Fisciano, Italy;14. Leeds Teaching Hospitals National Health Service Trust, Leeds, UK;15. Sapienza University of Rome, Rome, Italy;p. University College London, London, UK;q. University of Padua, Padua, Italy |
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| Abstract: | Subcutaneous implantation of demineralized bone matrix in allogenic rats induces a sequence of events resulting in de novo formation of cartilage, bone and bone marrow. In the present study endochondral bone formation by demineralized porcine matrix was studied and compared with the rat bone matrix. Endochondral bone formation was induced by 4M guanidine hydrochloride fraction IV (less than 50,000 daltons) of Sepharose CL-6B gel filtration but not by whole extract or by demineralized porcine bone matrix. Sephacryl S-200 gel filtration of the osteoinductive proteins of fraction IV showed the Porcine osteoinductive factor to be associated with protein fraction III (less than 20,000 daltons) whereas the rat with fraction II (between 20,000 and 30,000 daltons) of the chromatographic profile indicating an apparent difference in molecular weight of the osteoinductive factors between these two species. |
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