Enzyme-linked lectinosorbent assay of Escherichia coli and Staphylococcus aureus |
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Authors: | O. D. Hendrickson N. I. Smirnova A. V. Zherdev V. K. Gasparyan B. B. Dzantiev |
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Affiliation: | 1.A.N. Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences,Moscow,Russia;2.G.Kh. Bunatyan Institute of Biochemistry,National Academy of Sciences of Armenia,Erevan,Armenia |
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Abstract: | In this study, we developed a microplate sandwich analysis of Escherichia coli and Staphylococcus aureus bacterial pathogens based on the interaction of their cell wall carbohydrates with natural receptors called lectins. An immobilized lectin-cell-biotinylated lectin complex was formed in this assay. Here, we studied the binding specificity of several plant lectins to E. coli and S. aureus cells, and pairs characterized by high-affinity interactions were selected for the assay. Wheat germ agglutinin and Ricinus communis agglutinin were used to develop enzyme-linked lectinosorbent assays for E. coli and S. aureus cells with the detection limits of 4 × 106 and 5 × 105 cells/mL, respectively. Comparison of the enzyme-linked immonosorbent assay and the enzyme-linked lectinosorbent assay demonstrated no significant differences in detection limit values for E. coli. Due to the accessibility and universality of lectin reagents, the proposed approach is a promising tool for the control of a wide range of bacterial pathogens. |
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