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Effect of lipopolysaccharide O-side chains on the adhesiveness of <Emphasis Type="Italic">Yersinia pseudotuberculosis</Emphasis> to J774 macrophages as revealed by optical tweezers |
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| Authors: | A A Byvalov V L Kononenko I V Konyshev |
| Institution: | 1.Vyatka State University,Kirov, ]610000,Russia;2.Institute of Physiology, Komi Research Center, Ural Branch,Russian Academy of Sciences,Syktyvkar,Russia;3.Emanuel Institute of Biochemical Physics,Russian Academy of Sciences,Moscow,Russia;4.Dmitry Rogachev Federal Research Center of Pediatric Hematology, Oncology, and Immunology,Moscow,Russia |
| Abstract: | A method has been developed for the quantitative estimation of the binding force of a model microsphere with a eukaryocyte based on the optical trap in order to study the molecular mechanism of adhesion between an individual bacterium and a host cell. The substantial role of LPS O-side chains in the adhesiveness of Yersinia pseudotuberculosis 1b to J774 macrophages has been revealed with the use of a set of microspheres functionalized with lipopolysaccharide (LPS) preparations and antibodies with different specificities. The results indicate the significance of the O-antigen as a pathogenicity factor of Y. pseudotuberculosis in colonization of a macroorganism. The developed methodical approaches can be applied to the study of molecular mechanisms of the pathogenesis of pseudotuberculosis and other infectious diseases to improve antiepidemic service. |
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