Synthesis of high-molecular-mass polyhydroxybutyrate from methanol in <Emphasis Type="Italic">Methyloligella halotolerans</Emphasis> C2 |
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Authors: | V A Ezhov N V Doronina M N Shmareva Yu A Trotsenko |
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Institution: | 1.Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences,Pushchino, Moscow oblast,Russia |
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Abstract: | The influence of the concentrations of carbon and energy sources (methanol) and nitrogen (NH 4 + ) on the yield and molecular mass (Mm) of poly-β-hydroxybutyrate (PHB) synthesized by the novel methylotroph Methyloligella halotolerans C2 was investigated. It was shown that the maximum concentrations of NH 4 + and methanol in the medium for PHB biosynthesis were 0.15 g/L and 1 ± 0.2 mL/L, respectively. A unique high-molecular (8000–10000 kDa) PHB was obtained with NH 4 + and methanol concentrations of 0.12 g/L and 1.25 mL/L, respectively, in a fermenter. The main physicochemical and mechanical properties of the obtained bioplastic with a molecular mass of 10000 kDa were determined. |
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