Covalent immobilization of D-aminoacylase of strain Rhodococcus armeniensis AM6.1 and the characteristics of the biocatalyst |
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Authors: | A. A. Hambardzumyan A. V. Mkhitaryan A. M. Paloyan S. A. Dadayan |
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Affiliation: | 1.SPC “Armbiotechnology” NAS RA,Yerevan,Armenia |
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Abstract: | Immobilization of D-aminoacylase of the strain Rhodococcus armeniensis AM6.1 was carried out on the silochrome C-80 with a yield of enzymatic activity of 20%. The temperature and pH optima, thermal stability and dependence of thermal stability on pH for free and immobilized enzymes were compared. The possibility of using free and immobilized D-aminoacylases to produce D-amino acids from their racemic mixtures was demonstrated. |
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