Cardiac nonmyofibrillar proteins in copper-deficient rats |
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Authors: | Denis M. Medeiros Laura Shiry A. Jeannine Lincoln Lawrence J. Prochaska |
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Affiliation: | (1) Department of Human Nutrition and Food Management, The Ohio State University, 265 Campbell Hall, 1787 Neil Avenue, 43210-1295 Columbus, OH;(2) Department of Biochemistry, Wright State University, 45435 Dayton, OH |
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Abstract: | Cardiac nonmyofibrillar proteins from copper-deficient rats appear to have diminished quantity of selected peptides. Identification of some of these peptides was the objective of the present study. Male weanling Long-Evans rats were fed either copper-adequate (n=6) or copper-deficient (n=6) diets for 5 wk. At the end of 5 wk, the rat hearts were removed, quick frozen in liquid nitrogen, and non-myofibrillar proteins separated using sodium-dodecyl-sulfate poly-acrylamide gel electrophoresis (SDS-PAGE). A peptide in the 16-kDa mol-wt region was diminished in copper-deficient rats. Blotting of gels to an Immobilon-P membrane and subsequent sequencing of the amino acids identified the peptide as the δ subunit of mitochondrial ATP synthase. Blotting of gels to nitrocellulose followed by Western blot assay for cytochrome C oxidase using antibodies against the enzyme complex revealed decreased protein content in the copper-deficient rat for this enzyme, primarily the nuclear encoded subunits. |
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Keywords: | Heart ATP synthase cytochrome C oxidase copper deficiency rats |
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