The human intestinal cell lines Caco-2 and LS174T as models to study cell-type specific mucin expression |
| |
Authors: | B Jan-Willem van Klinken Esmeralda Oussoren Jan-Johan Weenink Ger J Strous Hans A Büller Jan Dekker Alexandra W C Einerhand |
| |
Institution: | (1) Pediatric Gastroenterology and Nutrition, Academic Medical Center, Room G8-260, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands;(2) Department of Cell Biology, Medical School, Utrecht, The Netherlands |
| |
Abstract: | Mucin expression was studied during proliferation and differentiation of the enterocyte-like Caco-2 and goblet cell-like LS174T cell lines. Caco-2 cells express mRNAs of MUC1, MUC3, MUC4 and MUC5A/C whereas MUC2 and MUC6 mRNAs are virtually absent. Furthermore, MUC3 mRNA is expressed in a differentiation dependent manner, as is the case for enterocytes. Concomitantly MUC3 protein precursor ( 550 kDa) was detected in Caco-2 cells. In LS174T cells mucin mRNAs of MUC1, MUC2 and MUC6 are constitutively expressed at high levels, whereas MUC3, MUC4 and MUC5A/C mRNAs are present at low levels. At the protein level LS174T cells express the goblet cell specific mucin protein precursors MUC2, MUC5A/C and MUC6 with apparent molecular masses of about 600 kDa, 470/500 kDa and 400 kDa respectively. MUC3 protein is not detectable. Furthermore, human gallbladder mucin protein ( 470 kDa precursor), of which the gene has not yet been identified, is expressed in LS174T cells. In addition, synthesis and secretion of the goblet cell specific mature MUC2, MUC5A/C and human gallbladder mucin was demonstrated in LS174T cells. It is concluded that Caco-2 and LS174T cell lines provide excellentin vitro models to elucidate the cell-type specific mechanisms responsible for mucin expression.Abbreviations SDS-PAGE
sodium dodecylsulfate-polyacrylamide gel electrophoresis
- DMEM
Dulbecco's modified Eagle's medium
- EMEM
Eagle's minimum essential medium
- Endo-H
endo- -N-acetylglucosaminidase H
- HGBM
human gallbladder mucin
- dpc
days past confluence
- PBS
phosphate buffered saline |
| |
Keywords: | mucin-type glycoproteins MUC1-6 mRNA biosynthesis protein biosynthesis Caco-2 LS174T human intestine |
本文献已被 SpringerLink 等数据库收录! |
|