Specificity of the isolectins from the plant cactusMachaerocereus eruca for oligosaccharides from porcine stomach mucin |
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| Authors: | Edgar Zenteno Lorena Vázquez Raúl Chávez Félix Córdoba Jean Michel Wieruszeski Jean Montreuil Henri Debray |
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| Affiliation: | (1) Laboratorio Biología Experimental, Centro de Investigaciones Biológicas J. Félix Frías, Universidad Autónoma del Estado de Morelos, 68290 Cuernavaca Morelos, México;(2) Departmento de Bioquímica Facultad de Medicina UNAM, 04510, México;(3) Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, 14080 Tlalpan, D.F., México;(4) Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq CEDEX, France;(5) Dep Bioquímica, Fac Medicina UNAM, P.O. Box 70159, 04510, México DF |
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| Abstract: | Sugar specificity of theMachaerocereus eruca isolectins, MeAI and MeAII, has been determined by comparing the capacity of glycans with well defined structures to inhibit their haemagglutinating activity. Both are galactose-specific isolectins with high affinity for O-glycans. However, the twoM. eruca isolectins recognize different oligosaccharidic sequences belonging to O-glycosidically linked glycans from porcine stomach mucin. The minimal structure recognized by MeAI on the porcine mucin glycans is the O-glycan core Gal 1, 3GalNAc-ol, whereas MeAII has a more extended site and interacts with a biantennary O-glycan possessing the terminal trisaccharide Fuc 1,2 (GalNAc1,3) Gal1,4. |
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| Keywords: | cactus lectins mucin specific lectins glycoproteins plant lectins |
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