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Préparation et dosage immunonéphélémétrique du fibrinogène de lapin
Authors:Didier Dhermy   Claudine Judox   Robert Engler  Max-F. Jayle  
Abstract:Pure rabbit fibrinogen was prepared by a method involving two ammonium sulfate precipitations, one 2 M phosphate buffer precipitation, one DEAE cellulose chromatography and lastly one Sepharose 6 B chromatography. The aminoacid composition was determined and an immunonephelemetric assay was proposed. This assay allowed an accurate determination of fibrinogen concentration in a rabbit with inflammatory reaction.
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