Construction and characterization of the IGF Arabidopsis BAC library |
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| Authors: | T Mozo S Fischer H Shizuya and T Altmann |
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| Institution: | (1) Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195 Berlin, Germany, DE;(2) Max-Planck-Institut für Molekulare Pflanzenphysiologie, Dept. Willmitzer, Karl-Liebknecht-Str. 25, Haus 20, D-14476 Golm, Germany E-mail: altmann@mpimp-golm.mpg.de Tel.: +49-331-9772780, Fax: +49-331-9772301, DE;(3) California Institute of Technogy, Pasadena, CA 91125, USA, US |
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| Abstract: | A bacterial artificial chromosome (BAC) library has been established for Arabidopsis thaliana (ecotype Col-0) covering about seven haploid nuclear genome equivalents. This library, called the Institut für Genbiologische
Forschung (IGF) BAC library, consists of 10 752 recombinant clones carrying inserts (generated by partial EcoRI digestion) of an average size of about 100 kb in a modified BAC vector, pBeloBAC-Kan. Hybridization with organellar DNA
and nuclear repetitive DNA elements revealed the presence of 1.1% clones with mitochondrial DNA, 0.2% clones with plastid
DNA, 3.2% clones with the 180 bp paracentromeric repeat, 1.6% clones with 5S rDNA, and 10.8% clones with the 18S-25S rDNA
repeat. With its extensive genome coverage, its rather uniformly sized inserts (80 kb <85% <120 kb) and low contamination
with organellar DNA, this library provides an excellent resource for A. thaliana genomic mapping, map-based gene cloning, and genome sequencing.
Received: 26 November 1997 / Accepted: 19 February 1998 |
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| Keywords: | Arabidopsis thaliana Genomic library Bacterial artificial chromosome pBeloBAC-Kan Repetitive sequence |
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