In vitro antioxidant profile of phenolic acid derivatives |
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Authors: | Cos Paul Rajan Padinchare Vedernikova Irina Calomme Mario Pieters Luc Vlietinck Arnold J Augustyns Koen Haemers Achiel Vanden Berghe Dirk |
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Affiliation: | a Laboratory of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.b Laboratory of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.c Laboratory of Pharmacognosy, Faculty of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. |
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Abstract: | Several caffeic acid esters isolated from propolis exhibit interesting antioxidant properties, but their in vivo use is compromised by hydrolysis of the ester bond in the gastrointestinal tract. Therefore, a series of caffeic acid amides were synthesized and their in vitro antioxidant profile was determined. A series of hydroxybenzoic acids, hydroxycinnamic acids, and the synthesized caffeic acid amides were tested for both their 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and microsomal lipid peroxidation-inhibiting activity. Some of the highly active antioxidants were further tested by means of electron paramagnetic resonance for their hydroxyl radical scavenging activity. Since a promising antioxidant compound should show a lipid peroxidation-inhibiting activity at micromolar level and a low cytotoxicity, the cytotoxicity of the phenolic compounds was also studied. In all the assays used, the caffeic acid anilides and the caffeic acid dopamine amide showed an interesting antioxidant activity. |
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Keywords: | Caffeic Acid Polyphenols Antioxidant Dpph Lipid Peroxidation Epr Spectroscopy |
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