A hypermorphic mutation in the protein phosphatase 2C HAB1 strongly affects ABA signaling in Arabidopsis |
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Authors: | Robert Nadia Merlot Sylvain N'guyen Vinh Boisson-Dernier Aurélien Schroeder Julian I |
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Institution: | Division of Biological Sciences, Cell and Developmental Biology Section, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116, USA. narobus@yahoo.com |
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Abstract: | Protein phosphatases of the 2C family (PP2C) function in the regulation of several signaling pathways from prokaryotes to eukaryotes. In Arabidopsis thaliana, the HAB1 PP2C is a negative regulator of the stress hormone abscisic acid (ABA) signaling. Here, we show that plants expressing a mutant form of HAB1 in which Gly246 was mutated to Asp (G246D) display strong ABA insensitive phenotypes. Our results indicate that the G246D mutation has a hypermorphic rather than a dominant negative effect. The data suggest that this mutation localized in a conserved motif in the PP2C catalytic domain could be used in other PP2Cs to reveal their biological functions. |
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Keywords: | ABA abscisic acid ABI abscisic acid insensitive LEA late embryogenesis abundant PP2C protein phosphatase 2C WT wild-type |
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