A modified ferrozine method for the measurement of enzyme-bound iron |
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| Authors: | M D Davis S Kaufman S Milstien |
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| Affiliation: | 1. Novosibirsk State University, 630090 Novosibirsk, Russian Federation;2. V. V. Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russian Federation;3. B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 220072 Minsk, Belarus |
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| Abstract: | A general procedure for the determination of the iron content of enzymes by digestion with methanesulfonic acid to release protein-bound iron has been developed. This procedure replaces the tedious and potentially hazardous method of wet ashing with concentrated nitric-sulfuric-perchloric acids. The method has been used to determine the stoichiometry of iron for nanomole quantities of heme-iron proteins, iron-sulfur proteins, complex iron-sulfur proteins, as well as in phenylalanine hydroxylase, an enzyme with iron in an undetermined coordination. |
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