Tissue,cell culture and micropropagation of Mandevilla velutina,a natural source of a bradykinin antagonist |
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| Authors: | W. Handro E. I. S. Floh C. M. Ferreira M. P. Guerra |
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| Affiliation: | (1) Plant Tissue Culture Laboratory, Department of Botany, Institute of Biosciences, University of São Paulo, C.P. 11461, 05499 São Paulo SP, Brazil;(2) Present address: Department of Phytotechny, UFSC, 88000 Florianópolis, SC, Brasil |
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| Abstract: | Leaf, stem and root explants of Mandevilla velutina were cultured in vitro and produced vigorous callus in LS basal medium containing one auxin (2,4-D or NAA) plus BAP. Calli can be subcultured indefinitely with vigorous growth. Subculture of calli to NAA (1.0 mg/l) plus BAP (5.0 mg/l) caused profuse regeneration of shoots. Isolated shoots were rooted in basal medium plus NAA (5.0 mg/l) or IBA (8.0 mg/l). Rapidly growing cell suspensions can be easily obtained from friable callus cultured in liquid medium.Abbreviations LS Linsmaier & Skoog - 2,4-D 2,4 dichlorophenoxi-acetic acid - NAA  -naphthalene-acetic acid - GA3 gibberellic acid - IAA indole-3-acetic acid - BAP 6-benzylaminopurine - IBA indole-3-butyric acid |
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