Evolution of the adenosine triphosphate site and design of new inhibitors of DNA topoisomerases II

DNA topoisomerase II is required for chromosome segregation. The enzyme expresses itsbiological activity upon ATP hydrolysis into ADPand inorganic phosphate. Both ATP and dATP areequivalent substrates, but the 8-azidoadenosine5-triphosphate is a poor substrate comparing toATP for calf thymus DNA topoisomerase II. Thisresult is interesting and can be used for thedesign of new inhibitors or new bettersubstrates and furthermore for biocaptors.