ABCG2 is expressed in late spermatogenesis and is associated with the acrosome |
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Authors: | Christian Scharenberg,Nadja Mannowetz,Robert W. Robey,Philip Repges,Thomas Jä hn |
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Affiliation: | a Department of Anatomy and Cell Biology, Saarland University, Building 61, 66421 Homburg, Germany b Department of Hematology and Oncology, Philipps-University, 35037 Marburg, Germany c Center of Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA |
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Abstract: | An increasingly exploited strategy for the isolation of stem cells is based on the increased efflux of Hoechst 33342 lipophilic dye mediated by ABCG2, an ATP-binding cassette transporter which is highly expressed in various stem cells. We found ABCG2 expression to be present at later stages of spermatogenesis. Western blot analysis using an anti-ABCG2 antibody revealed expression of a 72 kDa band in mature sperm obtained from mice, rats, bulls or humans. Immunocytochemistry studies revealed acrosomal staining pattern of ABCG2 in spermatozoa. Experiments using the Hoechst 33342 ABCG2 substrate and the ABCG2-specific inhibitor FTC demonstrated efflux activity of ABCG2 in mature sperm. Incubation of sperm in capacitating medium in the presence of the ABCG2-inhibitor FTC resulted in decreased cholesterol depletion compared to sperm incubated in the absence of FTC. Our results demonstrate that ABCG2 is expressed at the acrosome in mature sperm. ABCG2 may thus serve to mediate cholesterol removal. |
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Keywords: | ABCG2 Spermatozoa Acrosome Cholesterol efflux |
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