Developmental changes in the regulation of calcium-dependent neurite outgrowth |
| |
Authors: | Yuko Arie Masumi Iketani Ken Takamatsu Yoshio Goshima |
| |
Affiliation: | a Departments of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Fuku-ura 3-9, Kanazawa-ku, Yokohama 236-0004, Japan b Departments of Opthalmology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan c Department of Physiology, Toho University School of Medicine, Tokyo 143-8540, Japan d Laboratory of Developmental Neurobiology, Brain Science Institute, RIKEN, Wako 351-0198, Japan |
| |
Abstract: | Intracellular calcium ions (Ca2+) have an essential role in the regulation of neurite outgrowth, but how outgrowth is controlled remains largely unknown. In this study, we examined how the mechanisms of neurite outgrowth change during development in chick and mouse dorsal root ganglion neurons. 2APB, a potent inhibitor of inositol 1,4,5-trisphosphate (IP3) receptors (IP3R), inhibited neurite outgrowth at early developmental stages, but not at later stages. In contrast, pharmacological inhibition with Ni2+, Cd2+, or dantrolene revealed that ryanodine receptor (RyR)-mediated Ca2+-induced Ca2+ release (CICR) was involved in neurite outgrowth at later stage, but not at early stages. The distribution of IP3R and RyR in growth cones also changed during development. Furthermore, pharmacological inhibition of the Ca2+-calmodulin-dependent phosphatase calcineurin with FK506 reduced neurite outgrowth only at early stages. These data suggest that the calcium signaling that regulates neurite outgrowth may change during development from an IP3R-mediated pathway to a RyR-mediated pathway. |
| |
Keywords: | Developmental changes Neurite outgrowth Calcium IP3 receptor Ryanodine receptor DRG Sensory neuron |
本文献已被 ScienceDirect 等数据库收录! |
|