Penicillin V production by Penicillium chrysogenum in the presence of Fe3+ and in low-iron culture medium |
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Authors: | Leiter E Emri T Gyémánt G Nagy I Pócsi I Winkelmann G Pócsi I |
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Institution: | (1) Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, 4010 Debrecen, Hungary;(2) Department of Biochemistry, Faculty of Science, University of Debrecen, 4010 Debrecen, Hungary;(3) Department of Inorganic and Analytical Chemistry, Faculty of Science, University of Debrecen, 4010 Debrecen, Hungary;(4) Institute of Microbiology and Biotechnology, University of Tübingen, 72076 Tübingen, Germany |
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Abstract: | Late-exponential-phasePenicillium chrysogenum mycelia grown in a complex medium possessed an intracellular iron concentration of 650 μmol/L (2.2±0.6 μmol per g mycelial
dry mass). This iron reserve was sufficient to ensure growth and antibiotic production after transferring mycelia into a defined
low-iron minimal medium. Although the addition of Fe3+ to the Fe-limited cultures increased significantly the intracellular iron levels the surplus iron did not influence the production
of penicillin V. Supplements of purified majorP. chrysogenum siderophores (coprogen and ferrichrome) into the fermentation media did not affect the β-lactam production and intracellular
iron level. Neither 150 nor 300 μmol/L extracellular Fe3+ concentrations disturbed the glutathione metabolism of the fungus, and increased the oxidative stress caused by 700 mmol/L
H2O2. Nevertheless, when iron was applied in the FeII oxidation state the oxidative cell injuries caused by the peroxide were significantly enhanced. |
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