Permeabilization of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus with Ethanol |
| |
Authors: | George A. Somkuti Mary E. Dominiecki Dennis H. Steinberg |
| |
Affiliation: | (1) U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA , US |
| |
Abstract: | Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus cultures were treated with ethanol and tested for viability and β-galactosidase activity. Exposure of the biomass of test cultures to 30%–55% ethanol (vol/vol) caused a 100% loss of viability and up to 15-fold increase in measurable β-galactosidase activity in both streptococci and lactobacilli. Ethanol-treated cell suspensions could be stored for up to 6 months without loss of enzyme activity. The nonviable permeabilized biomass of the more active S. thermophilus was used to achieve up to 80% hydrolysis of lactose in aqueous solutions and non-fat milk. Received: 28 July 1997 / Accepted: 30 September 1997 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|