Prediction of mutant activity and its application in molecular design of tumor necrosis factor-a |
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Authors: | TANG Weidong XI Tao WANG Bo GUO Donglin XU Xianxiu ZHU Dexu |
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Institution: | (1) Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093 Nanjing, China |
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Abstract: | Two models for prediction of the activity and stability of site-directed mutagenesis on tumor necrosis factor-α are established.
The models are based on straightforward structural considerations, which do not require the elaboration of sitedirected mutagenesis
on the protein core and the hydrophobic surface area by analyzing the pmperties of the mutated amino acid residues. The reliabilities
of the models have been tested by analyzing the mutants of tumor necrosis factor-α (TNF-α) whose two leucine residues (L29,
L157) were mutated. Based on these models, a TNFα mutant with high activity was created by molecular design.
Project supported by the Chinese National High Technology Development Program. |
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Keywords: | prediction model tumor neauwia factor-α molecular design |
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