Mapping cynomolgus monkey MHC class I district on chromosome 6p13 using pooled cDNAs |
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| Authors: | Qy Liu Xx Wang Jz Zhang Wh Chen Xw He Y Lin |
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| Institution: | 1. Beijing Institute of Genomics, Beijing, 101300, China;2. Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning, 530005, China;3. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510640, China |
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| Abstract: | The cynomolgus monkey (Macaca fascicularis) is a frequently used animal model for studying human diseases, especially immune related ones. For a better understanding of its major histocompatibility complex (MHC) class I district chromosome location, we selected seven cDNA clones as probes for fluorescence in situ hybridization (FISH) from a lymphocyte cell line cDNA library. Expressed sequence tags (ESTs) from these clones were assembled into three clusters and annotated Mafa-A and Mafa-B genes. Further bioinformatics analysis shows that they had multiple duplications spanning approximately 2.8 Mb on the rhesus macaque MHC class I district. Using the FISH technique, we mapped the seven pooled cDNA clones to the short arm of the cynomolgus monkey chromosome 6 on 6p13. To our knowledge, this is the first report of the location of cynomolgus monkey MHC class I district. Using pooled adjacent cDNAs as probes also allows affordable, specific genome region mapping research. |
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| Keywords: | cynomolgus FISH MHC class I pooled cDNAs 6P13 |
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