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The use of dimethylsulfoxide as a vehicle in cell culture experiments using ovarian carcinoma cell lines
Authors:C Rodríguez-Burford  D K Oelschlager  L I Talley  M N Barnes  E E Partridge
Institution:1. Department of Pathology,;2. Comprehensive Cancer Center Biostatistics Unit,;3. Department of Obstetrics and Gynecology, The University of Alabama at Birmingham, Birmingham, Alabama, 35233-0007
Abstract:We describe here a computational morphology-based approach to the investigation of possible causes of chromatin alterations in sperm. A comprehensive set of state-of-the-art and geometric measures are computationally extracted from toluidine blue stained images and analyzed to infer the possible processes leading to normal and abnormal chromatin formation while seeking a possible taxonomy of chromatin alterations and their influence on sperm head morphology. Using this methodology, we have identified higher chromatin fragility at some specific points of the sperm head. Despite the lack of correlation between morphologies of sperm head and chromatin structure, four main morphological types of chromatin alterations in bull spermatozoa have been identified and their possible causes discussed.
Keywords:chromatin  computational biology  germ cell  image cytometry  spermatozoa
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