The relation of oxidative stress and apoptosis to histopathologic alterations in the lungs as a result of global cerebral ischemia |
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Authors: | H. Yeşil İ. Tuğlu |
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Affiliation: | 1. Departments of Midwifery, Celal Bayar University Manisa, Manisa, Turkeynisalisayy@hotmail.com https://orcid.org/0000-0002-9790-1723;3. Histology and Embryology, Faculty of Medicine, Celal Bayar University Manisa, Manisa, Turkey https://orcid.org/0000-0002-0569-8415 |
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Abstract: | ABSTRACTHeart attack and oxygen deficiency may cause necrosis in the brain and other tissues. We investigated the histopathological effects of nitric oxide (NO) on ischemia/reperfusion in lung and hippocampus using a rat brain bilateral occlusion ischemia model. Male rats were assigned to sham (SH), ischemic preconditioning (PC), global ischemia (GI) and ischemic reperfusion (IR) groups. Before ischemia was induced, blood was drawn to induce hypovolemic hypotension and for blood gas testing. After sacrifice, samples of hippocampus were harvested. Sections were examined using hematoxylin and eosin (H & E) staining and immunostaining using primary antibodies for GFAP, S100β, iNOS, eNOS and the TUNEL method. Following ischemia, we found evidence of gliosis induced oxidative stress and apoptosis in the hippocampus. No significant differences were detected between the SH and PC groups. In the GI and IR groups, apoptosis and necrosis were observed in the hippocampus. Lung sections were stained with H & E and Masson’s trichrome (MT) and immunostained for iNOS and eNOS. The TUNEL method was used to detect apoptosis. Interstitial edema, vascular congestion, intra-alveolar hemorrhage, perivascular edema, neutrophil infiltration and disruption of alveoli were observed after global ischemia and ischemic reperfusion. Inflammatory cells were detected in the connective tissue. The IR and GI groups exhibited significantly more apoptotic cells than the SH or PC groups. Free radicals, such as nitric oxide (NO), that appear following ischemia and reperfusion in the brain may also injure the lungs. Increased NO in both lung and brain tissue suggests that apoptosis in these organs can be induced by reactive nitrogen species. |
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Keywords: | Apoptosis brain histology immunohistochemistry ischemia lung oxidative stress rats reperfusion |
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