Involvement of calcium in the in vitro sensitivity change of the plasma membrane H+ ATPase to indole-3-acetic acid |
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Authors: | Jalil Bellamine Hubert Greppin |
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Institution: | (1) Laboratory of Plant Physiology and Biochemistry, 3 place de l’Université, CH-1211 Genève 4, Switzerland |
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Abstract: | The proton pumping activity of phase-partitioning purified plasma membrane fraction from spinach leaves was tested in vitro in the presence of exogenous indole-3-acetic acid. The sensitivity of the H+ pumping activity to the auxin was changed after
flowering induction. We investigated the effect of whole spinach leaf treatments with substances affecting the phosphatidylinositol
diphosphate transduction pathway on the in vitro sensitivity modification by photoperiodic induction. A role of calcium ions was supported by studies on leaves treated with
a specific Ca2+ chelator (EGTA), a synthetic Ca2+ ionophore (A23187) or with calcium channel blokers (verapamil, lanthan chloride). An experiment using the transduction pathway
inhibitor, lithium chloride, indicated that the intracellular concentration of Ca2+ was increased by inositol triphosphate. |
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Keywords: | Auxin H+-ATPase Photoperiodic induction Plasmalemma Spinacia oleracea |
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