| 共代谢条件下光合细菌对2-氯苯酚的生物降解 |
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| 作者姓名： | Dong YH Hu XM He YD Li L |
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| 作者单位： | 1. 沈阳大学生物与环境工程学院,沈阳,110044;东北大学资源与土木工程学院,沈阳,110004
2. 东北大学资源与土木工程学院,沈阳,110004 |
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| 基金项目： | 国家水体污染控制与治理科技重大专项 |
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| 摘 要： | 光合细菌PSB-1D不能利用2-氯苯酚(2-CP)作为唯一的碳源和能源.选用苹果酸、丙酸钠、乙酸钠、柠檬酸钠、苯酚、葡萄糖和可溶性淀粉等7种不同碳源作为光合细菌PSB-1D降解2-CP的共代谢基质,考察了在黑暗好氧培养条件下,不同共代谢基质对PSB-1D生长及降解2-CP效果的影响.结果表明:葡萄糖能够很好地促进PSB-1D的大量繁殖,提高降解效果,缩短降解周期,为最佳共代谢基质.对葡萄糖的投加浓度进行了优化,当葡萄糖的投加浓度为3 g·L-1时,菌株PSB-1D培养168 h后的菌体生长浓度△D560为1.749,2-CP的半衰期为3.9 d,降解速率常数为0.00864 h-1.采用SDS-PAGE对微生物全细胞蛋白质进行分析发现,在共代谢过程中当菌株PSB-1D利用葡萄糖作为底物提供能源和碳源时,可诱导产生2-CP特异性降解酶.
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| 关 键 词： | 光合细菌 2-氯苯酚 共代谢 生物降解 降解动力学 |
Biodegradation of o-chlorophenol by photosynthetic bacteria under co-metabolism |
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| Dong YH,Hu XM,He YD,Li L.Biodegradation of o-chlorophenol by photosynthetic bacteria under co-metabolism[J].Chinese Journal of Applied Ecology,2011,22(5):1280-1286. |
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| Authors: | Dong Yi-Hua Hu Xiao-Min He Ying-Dian Li Liang |
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| Institution: | DONG Yi-hua1,2,HU Xiao-min2,HE Ying-dian2,LI Liang2(1College of Biological and Environmental Engineering,Shenyang University,Shenyang 110044,China,2College of Resources and Civil Engineering,Northeastern University,Shenyang 110004,China) |
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| Abstract: | Photosynthetic bacterial strain PSB-1D cannot utilize o-chlorophenol(2-CP) as the sole carbon source for energy.In this paper,different carbon sources(malic acid,sodium propionate,sodium acetate,sodium citrate,phenol,glucose,and soluble starch) were taken as the co-metabolism substrates to study their effects on PSB-1D growth and 2-CP degradation under the condition of aerobic culture in darkness.Among the substrates,glucose was most efficient,which promoted the reproduction of PSB-1D,enhanced the 2-CP degr... |
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| Keywords: | photosynthetic bacteria o-chlorophenol co-metabolism biodegradation degradation kinetics |
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