Lactose-induced Production of Human Soluble B Lymphocyte Stimulator (hsBLyS) in E. coli with Different Culture Strategies |
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Authors: | Zhaopeng Li Xu Zhang Tianwei Tan |
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Affiliation: | (1) College of Life Science and Technology, Beijing University of Chemical Technology, 100029 Beijing, P.R. China |
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Abstract: | Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose after batch culture, thereby giving hsBLyS at 3.7 g l−1 and a productivity of 0.11 g l−1 h−1. Revisions requested 1 September 2005 and 11 November 2005; Revisions received 7 November 2005 and 4 January 2006 |
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Keywords: | Escherichia coli human soluble B lymphocyte stimulator lactose induction |
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