首页 | 本学科首页   官方微博 | 高级检索  
     


Lactose-induced Production of Human Soluble B Lymphocyte Stimulator (hsBLyS) in E. coli with Different Culture Strategies
Authors:Zhaopeng Li  Xu Zhang  Tianwei Tan
Affiliation:(1) College of Life Science and Technology, Beijing University of Chemical Technology, 100029 Beijing, P.R. China
Abstract:Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose after batch culture, thereby giving hsBLyS at 3.7 g l−1 and a productivity of 0.11 g l−1 h−1. Revisions requested 1 September 2005 and 11 November 2005; Revisions received 7 November 2005 and 4 January 2006
Keywords:Escherichia coli  human soluble B lymphocyte stimulator  lactose induction
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号