Somatic embryogenesis and plant regeneration in Quercus acutissima |
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Authors: | Yong Wook Kim Bong Choon Lee Suk Koo Lee Suk Sung Jang |
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Institution: | (1) Forest Genetics Research Institute, P.O. Box 24, Suwon, Kyeonggido, Republic of Korea |
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Abstract: | Immature embryos of Quercus acutissima were collected weekly beginning 5 weeks post-fertilization and cultured on modified MS(Murashige and Skoog) medium containing 1,000 mg/l glutamine and 5 mM proline with different combinations of IBA(0.5–10.0 mg/l) and BA(0 or 1.0 mg/l) in light. The highest percentage of embryogenic cultures occurred on the medium containing 0.5 mg/l IBA or 1.0 mg/l BA and 0.5 mg/l IBA. Four weeks after initiation, the embryogenic cultures were transferred to MS medium without plant growth regulators and cultured for 4 weeks. The somatic embryos were then transferred to germination medium. The best germination results were achieved from WPM(Woody Plant Medium) containing 0.1 mg/l BA. Plantlets from somatic embryos were incubated on WPM supplemented with 0.2 mg/l BA for 4 weeks and plantlets with well developed shoots and roots were transplanted to perlite and peat moss(11, v/v) mixtures and placed in a culture room. After being hardened off for 8 weeks, they were transferred outdoors where they grew.Abbreviation BA
N6-benzyladenine
- IBA
indole-3-butyric acid
- GA3
gibberellic acid
- ABA
abscisic acid
- MS
Murashige & Skoog Medium
- WPM
Woody Plant medium |
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Keywords: | Quercus acutissima Immature embryos Somatic embryos Germination Plant regeneration |
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