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An efficient method for the in vitro management of multiple garlic accessions
Authors:Xabier Barandiaran  Nieves Martín  Carmen Alba  María Fernanda Rodríguez-Conde  Antonio Di Pietro  Jesus Martín
Affiliation:(1) Departamento de Microbiología, Facultad de Ciencias Universidad de Córdoba, C/. San Alberto Magno s/n, Córdoba, Spain;(2) Departmento de Genética, Facultad de Ciencias, Universidad de Córdoba, C/. San Alberto Magno s/n, Córdoba, Spain
Abstract:Summary A simple protocol for garlic in vitro management is described. It comprises a single medium for all developmental stages and genotypes tested, and the use of immature bulbs as source of axillary buds. Although genetic variability exists among the different accessions tested, for both the multiplication rate and bulblet size, acceptable values of multiplication were reached for all the accessions, and values of bulb formation approached 100% in the shoots produced.
Keywords:Allium sativum   micropropagation  bulb induction  genetic variability  tissue culture
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