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Plant Cuticle Staining with Bismarck Brown Y and Azure B or Toluidine Blue O before Paraffin Extraction |
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| Authors: | Effin T Graham Priyavadan A Joshi |
| Institution: | a Department of Ornamental Horticulture, The University of Tennessee, Knoxville, Tennessee |
| Abstract: | Transverse paraffin sections of mature greenwood stems of rose (Rosa x hybrida) and flowering dogwood (Cornus florida L.) were stained with Bismarck brown followed by azure B or tolnidine blue O. The Bismarck brown was replaced by thiazin dye metachromasia in all structures except the cuticle which remained brown or yellow. The interface between the cuticle and exterior cell walls of the epidermis was delineated clearly. |
| Keywords: | rose Rosa x hybrida dogwood Cornus florida mature greenwood stem Bismarck brown T azure B tolnidine blue O benzoate buffer cuticle metachromasia |
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