银杏愈伤组织培养及其代谢产物银杏内酯的研究

筛选出了愈伤组织生长的最佳培养基；考察了各种理化因子对培养细胞生长及银杏内酯产生的影响；应用生物法和HPLC对愈伤组织中的银杏内酯A和B进行了成功的测试。结果显示，银杏愈伤组织培养物中银杏内酯的含量可达0.01%，属国际领先水平。

Studies on the Callus Culture of Ginkgo biloba and its Metabolites ginkgolides

The production of Ginkgolides in callus culture of Ginkgo biloba was reported. The affection of some physical factors and chemical substances on the induction and growth of calli was also investigated. A biologically quantitative method (platelet aggregation induced by PAF) and HPLC were successfully used for the determination of Ginkgolides A and B in all kinds of callus cultures. The result showed that the content of Ginkgolide B in the callus cultures varies from 0.005% to 0.01%, which is one of the best result for the callus culture of G.biloba in the world.