| 稀有密码子对proUK基因在大肠杆菌中高表达的影响 |
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| 引用本文： | 蒋岚 杨永华. 稀有密码子对proUK基因在大肠杆菌中高表达的影响[J]. 生物工程学报, 1999, 15(1): 64-67 |
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| 作者姓名： | 蒋岚 杨永华 |
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| 作者单位： | 中国科学院上海生物工程研究中心 |
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| 基金项目： | 国家高技术研究发展计划 |
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| 摘 要： | ＡＧＧ／ＡＧＡ密码子在ｐｒｏＵＫ基因中出现的频率高达２％，大肠杆菌中现有的编码ｔＲＮＡＵＣＵ的ａｒｇＵ基因不能满足ｐｒｏＵＫ高表达的需要。本文研究了ａｒｇＵ基因剂量对ｐｒｏＵＫ表达的影响，结果表明带有ａｒｇＵ基因的中等拷贝数质粒能促进ｐｒｏＵＫ基因表达。
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| 关 键 词： | argU，稀有密码子，proUK |
The Rare Coden Effects the Overexpression of Human proUK Gene in Escherichia coli |
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| Jiang Lan Yang Yonghua Gong Yi Yang Shengli. The Rare Coden Effects the Overexpression of Human proUK Gene in Escherichia coli[J]. Chinese journal of biotechnology, 1999, 15(1): 64-67 |
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| Authors: | Jiang Lan Yang Yonghua Gong Yi Yang Shengli |
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| Abstract: | The usage frequency of coden AGG and AGA in human proUK gene is reached to 2%, which causes the amount of tRNAucu from chromosomal argU gene to be limit factor for translation of proUK mRNA, This paper reports the relationship between argU gene doses and proUK mRNA translational efficiency. The results indicated that a kind of mid copy number plasmid with the argU gene can increase the expression of the proUK gene. |
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| Keywords: | argU rare coden proUK |
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