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Designing Out Disulfide Bonds of Leech Carboxypeptidase Inhibitor: Implications for Its Folding, Stability and Function

Authors:	Joan L Arolas Salvador Ventura

Institution:	Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Barcelona, Spain Departament de Bioquímica i Biologia Molecular, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Barcelona, Spain

Abstract:

Keywords:	bis-ANS 4 4'-bis(1-anilinonaphthalene-8-sulfonate) BPTI bovine pancreatic trypsin inhibitor CPA carboxypeptidase A GdnHCl guanidine hydrochloride GSH reduced glutathione GSSG oxidized glutathione K_i inhibition constant LCI leech carboxypeptidase inhibitor MALDI-TOF MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry MCP metallocarboxypeptidase RP-HPLC reversed-phase high-performance liquid chromatography TFA trifluoroacetic acid WT wild type
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